Home	Antibody sequencing	Recombinant antibodies	Affinity maturation	Framework grafting
Humanization and Framework Grafting Services Antibody humanization (from $2,500/gene, 4-6 weeks, please inquire) Client provides sequence information and desirable antibody format. We will analyze the sequences, identify the best matching human frameworks, design human equivalents, assemble them using our block-based gene assembling technology and clone into our bicistronic mammalian expression vector. We deliver ~10μg of spin-column purified plasmid DNA and detailed project report containing the plasmid map, sequence trace files and annotated sequences of the expression cassettes. We recommend using this in combination with our affinity maturation service. Framework grafting (from $2,500/gene, 4-8 weeks, please inquire) Client provides sequence information and desirable antibody format. We will analyze the sequences, identify the best matching frameworks from the available frameworks of the species specified by the Client, design synthetic equivalents, assemble them using our block-based gene assembling technology and clone into our bicistronic mammalian expression vector. We deliver ~10μg of spin-column purified plasmid DNA and detailed project report containing the plasmid map, sequence trace files and annotated sequences of the expression cassettes. We recommend using this in combination with our affinity maturation service.